2018 EADV-ESDR SUMMER RESEARCH WORKSHOP

2018 EADV-ESDR SUMMER RESEARCH WORKSHOP

04/06/2018 - 08/06/2018

Title: Advanced molecular biology tools in dermatological research



Title: Advanced molecular biology tools in dermatological research

This workshop was led by Prof Caterina Missero with faculty from ESDR and was specifically aimed at dermatology residents/trainees and/or at PhD students and postdocs affiliated to dermatological research with limited experience in experimental research and in molecular biology.

The workshop comprised a mixture of research presentations, interactive and practical sessions. A booklet of methods and protocol in advanced molecular biology was given to participants during the course.

Topics covered included:

Gene transduction and silencing in epidermal cells:

  • isolation of human and mouse primary keratinocytes
  • keratinocyte culture and keratinocyte/fibroblast co-culture
  • efficient transduction of genes or gene knockdown in keratinocytes

Gene expression in skin in health and disease:

  • RNA isolation from human and mouse primary keratinocytes, from freshly isolated epidermis, and cells of interest from fixed skin
  • quantitative RT-PCR: procedure and data analysis
  • RNA sequencing and data analysis

Genomic editing:

  • designing of gene deletion or gene replacement by CRISPR/Cas9
  • preparation of Cas9-guide RNA vectors
  • assess genome editing efficiency
  • isolation of genome-edited cells